Primer

Part:BBa_J133700

Designed by: Norman Wang   Group: Presting Lab   (2011-06-24)


primers tail extension EX for BioBricking

Extend your cloning primers with BioBrick Prefix cutting sites (EcoRI-NotI-XbaI) by concatenating this sequence on the 5' end. Sequence is designed to minimize primer homodimers with itself, and heterodimer with BBa_J133701.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3
    Illegal NotI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3
    Illegal XbaI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None